Friday, September 6, 2013

Bacterial Genetics

  • Frequent question on the boards: 2 bacteria + Antibiotic resistant + DNAse = No grow is trans-FORMATION

1 comment:

  1. Q: Bacterial strain X is resistant to ampicillin and sensitive to gentamicin. Bacterial strain Y is resistant to gentamicin and sensitive to ampicillin. Bacterial strains X and Y are grown in mixed culture in medium without antibiotics, and then the culture is plated on medium containing both ampicillin and gentamicin. Bacterial colonies grow on the plates. In a second experiment, separate strains of X and Y are plated together on culture medium containing ampicillin, gentamicin, and DNAse. No colonies grow on these plates. Assuming that bacterial cells are impermeable to DNAse, what process best explains these observations?

    A: Transformation